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Accumulation of 210 kDa microtubule‐interacting protein in differentiating P19 embryonal carcinoma cells
Author(s) -
Zı́ková Martina,
Sulimenko Vadym,
Dráber Pavel,
Dráberová Eduarda
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01488-5
Subject(s) - p19 cell , thermolabile , retinoic acid , tubulin , neurofilament , antigen , embryonal carcinoma , incubation , biology , microbiology and biotechnology , microtubule , chemistry , cellular differentiation , biochemistry , immunohistochemistry , immunology , gene , enzyme , adult stem cell
The MA‐01 antigen, a thermolabile 210 kDa microtubule‐interacting protein, is present in P19 embryonal carcinoma cells on microtubular structures as well as in cytosol. After aggregation of the cells and subsequent incubation with all‐ trans ‐retinoic acid (RA), the level of MA‐01 expression increased approximately 10 times during 15 days. The increase started after 2 days of incubation with RA and preceded the appearance of neuron‐specific tubulin βIII, MAP2C and neurofilaments. Such elevated expression of MA‐01 antigen was not detected in P19 cells treated with dimethylsulfoxide. These data indicate that enhanced expression of MA‐01 antigen is one of the earliest events occurring in P19 cells during neuronal differentiation.