Premium
Solitary HERV‐K LTRs possess bi‐directional promoter activity and contain a negative regulatory element in the U5 region
Author(s) -
Domansky Andrey N.,
Kopantzev Eugene P.,
Snezhkov Eugene V.,
Lebedev Yuri B.,
Leib-Mosch Christine,
Sverdlov Eugene D.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01460-5
Subject(s) - reporter gene , long terminal repeat , promoter activity , promoter , retinoic acid , gene , teratocarcinoma , microbiology and biotechnology , response element , transcription (linguistics) , regulatory sequence , biology , silencer , transcription factor , gene expression , genetics , cellular differentiation , mechanical engineering , linguistics , philosophy , engineering , inlet
Reporter gene analysis of HERV‐K solitary long terminal repeats (LTRs) showed that they retain detectable activity in human teratocarcinoma cells, and can direct the transcription in both orientations relative to the reporter gene. Deletion analysis demonstrated the possible existence of alternative promoters within the LTR as well as a silencer‐like element in the U5 region. Our results indicate also that all‐ trans ‐retinoic acid is capable of modulating expression of the reporter gene directed by a HERV‐K LTR in NT2/D1 cells.