Premium
Targeting of the gene product encoded by ORF UL56 of human cytomegalovirus into viral replication centers
Author(s) -
Giesen Kyra,
Radsak Klaus,
Bogner Elke
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01407-1
Subject(s) - human cytomegalovirus , viral replication , dna replication , virology , biology , replication factor c , dna replication factor cdt1 , replication (statistics) , control of chromosome duplication , origin recognition complex , gene , dna , cytomegalovirus , eukaryotic dna replication , microbiology and biotechnology , virus , genetics , herpesviridae , viral disease
The highly conserved DNA‐binding protein pUL56 of human cytomegalovirus (HCMV) was found to be predominantly localized throughout the nucleus as well as in viral replication centers of infected cells. The latter localization was abolished by phosphono acetic acid, an inhibitor of viral DNA replication. Immunofluorescence revealed that pUL56 co‐localized in replication centers alongside pUL112–113 and pUL44 at late times of infection. By co‐immunoprecipitations, a direct interaction with pUL44, a protein of the replication fork, was detected. These results showed for the first time that HCMV pUL56 is localized in viral replication centers, implicating that DNA replication is coupled with packaging.