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Rad24 is essential for proliferation of diploid cells in fission yeast
Author(s) -
Tanaka Yoshimi,
Okuzaki Daisuke,
Yabuta Norikazu,
Yoneki Takahiro,
Nojima Hiroshi
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01395-8
Subject(s) - schizosaccharomyces pombe , gene , microbiology and biotechnology , ploidy , biology , cyclin dependent kinase 1 , protein kinase a , yeast , meiosis , schizosaccharomyces , phosphorylation , genetics , saccharomyces cerevisiae , cell cycle
The rad24 + gene of Schizosaccharomyces pombe encodes a ubiquitously expressed 14‐3‐3 protein. We report here that Δ rad24 cells displayed a defect in diploid colony formation, although they conjugated efficiently. We found that a cumulative deletion of mei2 + gene almost completely suppressed this defect, and demonstrated using two‐hybrid analysis that Rad24 protein directly associates with Mei2 protein by recognizing Ser‐438 which is a phosphorylation target of Pat1 kinase. We conclude that constitutive progression to meiosis, caused by lack of Mei2 inhibition due to the absence of Rad24 protein, is the primary cause of the proliferative deficiency observed in Δ rad24 cells.