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Cloning and expression of human TRAAK, a polyunsaturated fatty acids‐activated and mechano‐sensitive K + channel
Author(s) -
Lesage Florian,
Maingret François,
Lazdunski Michel
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01388-0
Subject(s) - arachidonic acid , polyunsaturated fatty acid , complementary dna , open reading frame , chemistry , microbiology and biotechnology , amino acid , quinidine , cloning (programming) , tetraethylammonium , biochemistry , gene , fatty acid , biology , peptide sequence , potassium , organic chemistry , computer science , programming language , pharmacology , enzyme
The two P domain hTRAAK K + channel has been cloned from human brain. hTRAAK cDNA encodes a 393 amino acid polypeptide with 88% of homology with its mouse counterpart. The hTRAAK gene has been mapped to chromosome 11q13 and the study of its organization indicates that the hTRAAK open reading frame is contained in six exons. hTRAAK is expressed abundantly in brain and placenta. In COS cells, hTRAAK currents are K + ‐selective, instantaneous and non‐inactivating. These currents are insensitive to the classical K + channels blockers 4‐aminopyridine, tetraethylammonium, barium and quinidine, but are strongly stimulated by application of arachidonic acid as well as other polyunsaturated fatty acids. hTRAAK can also be activated by a stretch of the membrane.