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Chlorophyll fluorescence quenching in isolated light harvesting complexes induced by zeaxanthin
Author(s) -
Wentworth Mark,
Ruban Alexander V.,
Horton Peter
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01369-7
Subject(s) - violaxanthin , antheraxanthin , xanthophyll , chlorophyll fluorescence , photochemistry , zeaxanthin , photosystem ii , quenching (fluorescence) , non photochemical quenching , lutein , chemistry , fluorescence , biophysics , chlorophyll , photosynthesis , biology , carotenoid , biochemistry , optics , physics , organic chemistry
Non‐photochemical quenching of chlorophyll fluorescence in plants occurs in the light harvesting antenna of photosystem II and is regulated by the xanthophyll cycle. A new in vitro model for this process has been developed. Purified light harvesting complexes above the detergent critical micelle concentration have a stable high fluorescence yield but a rapidly inducible fluorescence quenching occurs upon addition of zeaxanthin. Violaxanthin was without effect, lutein and antheraxanthin induced a marginal response, whereas the violaxanthin analogue, auroxanthin, induced strong quenching. Quenching was not caused by aggregation of the complexes but was accompanied by a spectral broadening and red shift, indicating a zeaxanthin‐dependent alteration in the chlorophyll environment.