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Human glutathione dependent prostaglandin E synthase: gene structure and regulation
Author(s) -
Forsberg Lena,
Leeb Lisa,
Thorén Staffan,
Morgenstern Ralf,
Jakobsson Per-Johan
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01367-3
Subject(s) - microbiology and biotechnology , gene , glutathione , exon , promoter , phenobarbital , gene expression , regulation of gene expression , psychological repression , chemistry , biology , enzyme , biochemistry , endocrinology
A P1 clone containing the gene for human glutathione dependent PGE synthase (PGES) was isolated and characterized. The gene is divided into three exons, spans 14.8 kb and was localized to chromosome 9q34.3. In A549 cells, the protein and activity levels of PGES were increased by interleukin‐1β. This increase was prevented by phenobarbital. Reporter constructs containing the 5′‐flanking region of exon 1, which exhibited strong promoter activity, responded accordingly, except that interleukin‐1β induced a transient increase followed by a decrease. As cyclooxygenase 2 expression has been reported to respond in a similar fashion, a transcriptional regulatory basis for the observed co‐regulation with PGES is implied. The strong down‐regulation by phenobarbital raises important issues concerning its mechanisms of action.