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Ligand interaction with the purified serotonin transporter in solution and at the air/water interface
Author(s) -
Faivre V.,
Manivet P.,
Callaway J.C.,
Airaksinen M.M.,
Morimoto H.,
Baszkin A.,
Launay J.M.,
Rosilio V.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01362-4
Subject(s) - serotonin transporter , imipramine , ligand (biochemistry) , serotonin plasma membrane transport proteins , monolayer , chemistry , serotonin , transporter , air interface , biophysics , molecule , stereochemistry , biochemistry , receptor , biology , organic chemistry , medicine , telecommunications , alternative medicine , pathology , computer science , wireless , gene
The purified serotonin transporter (SERT) was spread at the air/water interface and the effects both of its surface density and of the temperature on its interfacial behavior were studied. The recorded isotherms evidenced the existence of a stable monolayer undergoing a lengthy rearrangement. SERT/ligand interactions appeared to be dependent on the nature of the studied molecules. Whereas an unrelated drug (chlorcyclizine) did not bind to the spread SERT, it interacted with its specific ligands. Compared to heterocyclic drugs, for which binding appeared to be concentration‐dependent, a ‘two‐site’ mechanism was evidenced for pinoline and imipramine.