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A novel human processed gene, DAD‐R, maps to 12p12 and is expressed in several organs
Author(s) -
Kuittinen Tero,
Eggert Angelika,
Lindholm Päivi,
Horelli-Kuitunen Nina,
Palotie Aarno,
Maris John M.,
Saarma Mart
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01358-2
Subject(s) - biology , gene , open reading frame , complementary dna , microbiology and biotechnology , intron , genetics , peptide sequence
A cDNA of a processed gene of human DAD‐1 (defender against apoptotic cell death) was cloned from the human neuroblastoma cell line SH‐SY5Y. The genomic sequence of this novel processed gene, DAD‐R, lacked introns and was flanked by 8 bp terminal repeats. RT‐PCR showed that the transcript is expressed predominantly in testis, ovaries, pancreas, lung and skeletal muscle. DAD‐R has several possible initiation codons, one of them producing an open reading frame comprising 75% of the DAD‐1 gene. We determined the chromosomal localization of DAD‐R as 12p11.2–12p12.1, an area linked to familial synpolydactyly and frequently amplified in a variety of cancers, including those of testis, ovaries, pancreas and lungs.