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Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity
Author(s) -
Wang Xing Li,
Sim Ah Siew,
Wang Min Xia,
Murrell George A.C.,
Trudinger Brian,
Wang Jian
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01356-9
Subject(s) - enos , allele , medicine , genotype , nitric oxide synthase , nitric oxide synthase type iii , endothelial nitric oxide synthase , endocrinology , nitric oxide , enzyme , chemistry , biology , gene , biochemistry
We explored the interactive effects of endothelial nitric oxide synthase (eNOS) genotypes and cigarette smoking on protein levels and enzyme activity in 33 postpartum placentas. Whilst the eNOS protein levels were lower in the rare allele (0.48±0.11, n =9 vs. 1.05±0.10, n =24, P <0.01), the eNOS enzyme activity was about 7‐fold higher in the rare allele (4556.2±255.4 vs. 621.8±180.5 cpm/mg/min, P <0.01). Smokers had lower eNOS protein levels (1.07±0.09 vs. 0.50±0.19, P <0.05) in both alleles. It reduced the eNOS activities only in the rare allele (non‐smokers: 6143.8±251.2, n =5, smokers: 2968.5±259.4, n =4, 52% reduction, P <0.01). We conclude that associations between eNOS polymorphism and protein levels and enzyme activities are modifiable by smoking, the effects of smoking are dependent on the eNOS genotypes.