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A new endogenous differentiating factor (myelopeptide‐4) for myeloid cells
Author(s) -
Strelkov Leonid A,
Mikhailova Augusta A,
Fonina Larissa A,
Petrov Rem V
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01344-2
Subject(s) - lymphokine , myeloid , haematopoiesis , cell culture , microbiology and biotechnology , myeloid leukemia , cd14 , biology , monocyte , cellular differentiation , endogeny , cytoplasm , antigen , chemistry , biochemistry , immunology , flow cytometry , stem cell , gene , genetics
Along with known lymphokines involved in the regulation of hematopoiesis, a new differentiating factor (myelopeptide‐4, MP‐4) for myeloid cells was found. The peptide (Phe‐Arg‐Pro‐Arg‐Ile‐Met‐Thr‐Pro) originally isolated from the culture medium of porcine bone marrow cell culture was examined for its ability to induce differentiation in two human myeloid leukemia cell lines, HL‐60 and K‐562. Agents with well‐known differentiation‐inducing activity, such as phorbol myristate acetate, dimethylsulfoxide and the lymphokines were used as a reference. It has been shown that MP‐4 significantly influences the integral characteristics of metabolism, expression of surface antigens and morphology of these cells. It decreased the level of chromosomal DNA synthesis and, in parallel, increased the total protein synthesis in both HL‐60 and K‐562 cells. MP‐4 induced the expression of CD14 monocyte‐specific surface antigen and the appearance of mature monocytes/macrophages in HL‐60 cell cultures. There was a good correlation of cell metabolic/morphological changes and the CD14 marker expression for HL‐60 cells. A similar phenomenon was observed in K‐562 cells treated with MP‐4 when the levels of hemoglobin synthesis were detected in their cytoplasm. Thus, we consider MP‐4 as a new endogenous differentiating factor for myeloid cells.