Premium
In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2‐acetylaminofluorene/partial hepatectomy model in rats
Author(s) -
Shiota Goshi,
Kunisada Takahiro,
Oyama Kenji,
Udagawa Akihide,
Nomi Takahiro,
Tanaka Kiwamu,
Tsutsumi Atsushi,
Isono Masato,
Nakamura Takafumi,
Hamada Hirofumi,
Sakatani Takashi,
Sell Stewart,
Sato Kenzo,
Ito Hisao,
Kawasaki Hironaka
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01337-5
Subject(s) - hepatocyte growth factor , in vivo , hepatocyte , biology , medicine , hepatic stellate cell , growth factor , 2 acetylaminofluorene , endocrinology , cell growth , microbiology and biotechnology , messenger rna , chemistry , gene , in vitro , receptor , biochemistry , genetics , microsome
To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt–Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen‐positive hepatic oval cells were significantly elevated in HGF‐transferred rats, in which stem cell factor and c‐kit mRNA increased at each time point. Our results suggest that in vivo transfer of the HGF gene into liver accelerates proliferation of hepatic oval cells in the Solt–Farber model in rats.