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The survival motor neuron protein interacts with the transactivator FUSE binding protein from human fetal brain
Author(s) -
Williams Barbara Y,
Hamilton Susan L,
Sarkar Hemanta K
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01320-x
Subject(s) - transactivation , motor neuron , neuron , neuroscience , fetus , microbiology and biotechnology , fuse (electrical) , binding protein , biology , chemistry , biochemistry , physics , gene , transcription factor , genetics , pregnancy , quantum mechanics , spinal cord
To identify interacting proteins of survival motor neuron (SMN) in neurons, a fetal human brain cDNA library was screened using the yeast two‐hybrid system. One identified group of SMN interacting clones encoded the DNA transactivator FUSE binding protein (FBP). FBP overexpressed in HEK293 cells or endogenously expressed in fetal and adult mouse brain bound specifically in vitro to recombinant SMN protein. Furthermore, an anti‐FBP antibody specifically co‐immunoprecipitated SMN when both proteins were overexpressed in HEK293 cells. These results demonstrate that FBP is a novel interacting partner of SMN and suggests a possible role for SMN in neuronal gene expression.