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Polymyxin B binds to anandamide and inhibits its cytotoxic effect
Author(s) -
Wang Yin,
Liu Yan,
Sarker Krishna Pada,
Nakashima Mihoko,
Serizawa Takeshi,
Kishida Akio,
Akashi Mitsuru,
Nakata Masanori,
Kitajima Isao,
Maruyama Ikuro
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01313-2
Subject(s) - cytotoxic t cell , anandamide , chemistry , polymyxin b , pharmacology , polymyxin , biochemistry , medicine , cannabinoid receptor , in vitro , antibiotics , antagonist , receptor
Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to be a paracrine contributor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)‐immobilized beads column and eluted with ethanol. We confirmed the direct binding of PMB to ANA by using surface plasmon resonance. The adsorption of ANA by PMB may abolish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin shock.