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Effects of fatty acids, nucleotides and reactive oxygen species on durum wheat mitochondria
Author(s) -
Pastore Donato,
Fratianni Alessandra,
Di Pede Sergio,
Passarella Salvatore
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01292-8
Subject(s) - superoxide , reactive oxygen species , mitochondrion , linoleic acid , biochemistry , mitochondrial ros , chemistry , inner mitochondrial membrane , hydrogen peroxide , nucleotide , oxygen , fatty acid , biology , enzyme , gene , organic chemistry
Linoleic acid (LA) and other fatty acids added to respiring durum wheat mitochondria (DWM) were found to cause a remarkable membrane potential (Δ Ψ ) decrease, as monitored by measuring safranin fluorescence. The rate of Δ Ψ decrease showed (i) saturation dependence on LA concentration; (ii) fatty acid specificity; (iii) inhibition by externally added ATP, GDP, GTP and Mg 2+ and (iv) sigmoid dependence upon initial Δ Ψ , thus suggesting the existence of an active plant mitochondrial uncoupling protein (PUMP) in mitochondria from monocotyledonous species (durum wheat, Triticum durum Desf.). Surprisingly, the rate of the linoleate dependent Δ Ψ decrease was found to be activated by reactive oxygen species (ROS) (hydrogen peroxide and superoxide anion) and, moreover, linoleate proved to lower the mitochondrial generation of superoxide anion. These results suggest that ROS can activate PUMP, thus protecting the cell against mitochondrial ROS production.