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Profile of gene expression regulated by induced p53: connection to the TGF‐β family
Author(s) -
Kannan Karuppiah,
Amariglio Ninette,
Rechavi Gideon,
Givol David
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01291-6
Subject(s) - biology , gadd45 , gene , complementary dna , cyclin a , microbiology and biotechnology , ubiquitin ligase , cell cycle , cyclin d1 , cell cycle checkpoint , cancer research , genetics , ubiquitin
The transcription regulatory function of p53 was analyzed by using two inducible p53 systems in the human lung cancer cell line H1299. cDNA probes derived from RNA harvested 12 h after p53 induction were used to probe filters containing cDNA arrays. Over 20 genes were found to be significantly induced or suppressed by p53. The induced genes can be classified mainly as cell cycle inhibitors like p21waf, GADD45, apoptosis‐related genes like Fas/APO1 and PIG3 or DNA repair genes like DDB2, DNA ligase and G/T mismatch DNA glycosylase. The suppressed genes include mainly cell cycle regulators like cyclin B1, cyclin H and kinases like c‐abl, CLK1 and others. The most notable induced gene was MIC‐1, encoding a TGF‐β‐related secretory protein, suggesting a potential paracrine component for p53 growth suppression.