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Pertussis toxin prevents presynaptic inhibition by kainate receptors of rat hippocampal [ 3 H]GABA release
Author(s) -
A. Cunha Rodrigo,
Malva João O.,
Ribeiro J.A.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01272-2
Subject(s) - kainate receptor , metabotropic receptor , pertussis toxin , ionotropic effect , chemistry , receptor , pharmacology , g protein , biochemistry , glutamate receptor , biology , ampa receptor
Kainate receptors are ionotropic receptors, also reported to couple to G i /G o proteins, increasing neuronal excitability through disinhibition of neuronal circuits. We directly tested in hippocampal synaptosomes if kainate receptor‐mediated inhibition of GABA release involved a metabotropic action. The kainate analogue, domoate (3 μM), inhibited by 24% [ 3 H]GABA‐evoked release, an effect reduced by 76% in synaptosomes pre‐treated with pertussis toxin. Protein kinase C inhibition attenuated by 82% domoate‐induced inhibition of GABA release whereas protein kinase C activation did not change kainate receptor binding. Thus, domoate inhibition of GABA release recruits G i /G o proteins and a protein kinase C pathway.