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Molecular cloning of fresh water and deep‐sea rod opsin genes from Japanese eel Anguilla japonica and expressional analyses during sexual maturation 1
Author(s) -
Zhang Huan,
Futami Kunihiko,
Horie Noriyuki,
Okamura Akihiro,
Utoh Tomoko,
Mikawa Naomi,
Yamada Yoshiaki,
Tanaka Satoru,
Okamoto Nobuaki
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01233-3
Subject(s) - japanese eel , complementary dna , opsin , biology , gene , cloning (programming) , microbiology and biotechnology , southern blot , japonica , molecular cloning , genomic dna , genome , genetics , biochemistry , botany , rhodopsin , retinal , computer science , programming language
We have determined the complete cDNA sequences of fresh water rod opsin gene ( fwo ) and deep‐sea rod opsin gene ( dso ) from Japanese eel Anguilla japonica . The cDNA clones of fwo and dso consisted of 1437 and 1497 nucleotides, respectively. The predicted opsins of both genes consisted of 352 amino acid residues. Southern blot and PCR analyses of genomic DNA indicated that the Japanese eel genome contains only one fwo and one dso and they are intronless. Quantitative RT‐PCR analyses revealed that the expression of fwo decreases with sexual maturation while that of dso increases.