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cDNA cloning of turtle prion protein
Author(s) -
Simonic Tatjana,
Duga Stefano,
Strumbo Bice,
Asselta Rosanna,
Ceciliani Fabrizio,
Ronchi Severino
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01232-1
Subject(s) - complementary dna , cloning (programming) , turtle (robot) , coding region , biology , exon , prion protein , microbiology and biotechnology , gene , genetics , fishery , computer science , programming language , medicine , disease , pathology
Cloning of the cDNA coding for the 270‐residue turtle prion protein is reported. It represents the most remote example thus far described. The entire coding region is comprised in a single exon, while a large intron interrupts the 5′ UTR. The common structural features of the known prion proteins are all conserved in turtle PrP, whose identity degree to mammalian and avian proteins is about 40 and 58%, respectively. The most intriguing feature, unique to the turtle prion, is the presence of an EF‐hand Ca 2+ binding motif in the C‐terminal half of the protein.