Premium
Chronic ethanol ingestion increases efficiency of oxidative phosphorylation in rat liver mitochondria
Author(s) -
Piquet M.A,
Nogueira V,
Devin A,
Sibille B,
Filippi C,
Fontaine E,
Roulet M,
Rigoulet M,
Leverve X.M
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01225-4
Subject(s) - oxidative phosphorylation , cytochrome c oxidase , sodium azide , mitochondrion , ethanol , chemistry , phosphorylation , biochemistry , cytochrome , enzyme
The efficiency of oxidative phosphorylation was compared between rats chronically fed with ethanol and controls. (i) Results showed that the liver mitochondria state 4 respiratory rate was strongly inhibited, while the corresponding proton‐motive force was not affected; (ii) the cytochrome oxidase content and activity were decreased and (iii) the oxidative‐phosphorylation yield was increased in the ethanol exposed group. Furthermore, oxidative phosphorylation at coupling site II was not affected by ethanol. Cytochrome oxidase inhibition by sodium‐azide mimicked the effects of ethanol intoxication in control mitochondria. This indicates that the decrease in cytochrome oxidase activity induced by ethanol intoxication directly increases the efficiency of oxidative phosphorylation.