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Identification of lysophospholipid receptors in human platelets: the relation of two agonists, lysophosphatidic acid and sphingosine 1‐phosphate
Author(s) -
Motohashi Ken,
Shibata Satomi,
Ozaki Yukio,
Yatomi Yutaka,
Igarashi Yasuyuki
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01222-9
Subject(s) - lysophosphatidic acid , receptor , platelet , sphingosine , sphingosine 1 phosphate , g protein coupled receptor , autotaxin , lipid signaling , chemistry , protease activated receptor , agonist , platelet activation , microbiology and biotechnology , biochemistry , biology , immunology , thrombin
Lysophosphatidic acid (LPA) and sphingosine 1‐phosphate (Sph‐1‐P) are known as structurally related bio‐active lipids activating platelets through their respective receptors. Although the receptors for LPA and Sph‐1‐P have been recently identified in various cells, the identification and characterization of ones in platelets have been reported only preliminarily. In this report, we first investigated the distinct modes of LPA and Sph‐1‐P actions in platelet activation and found that LPA functioned as a much stronger agonist than Sph‐1‐P, and high concentrations of Sph‐1‐P specifically desensitized LPA‐induced intracellular Ca 2+ mobilization. In order to identify the responsible receptors underlying these observations, we analyzed the LPA and Sph‐1‐P receptors which might be expressed in human platelets, by RT‐PCR. We found for the first time that Edg2, 4, 6 and 7 mRNA are expressed in human platelets.