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Preparation and characterization of ‘heparinocytes’: erythrocytes with covalently bound low molecular weight heparin
Author(s) -
Müller Michele,
Büchi Linda,
Woodtli Karin,
Haeberli André,
Beer Jürg H
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01204-7
Subject(s) - heparin , chemistry , thrombin , covalent bond , cleavage (geology) , coagulation , hemolysis , low molecular weight heparin , substrate (aquarium) , anticoagulant , chromogenic , platelet , biochemistry , reagent , chromatography , immunology , organic chemistry , medicine , biology , paleontology , ecology , fracture (geology)
In an attempt to create the possibility of stable, long acting, intravascular anticoagulation, low molecular weight heparin was modified by introducing a sulfhydryl group into the molecule (LMWH‐SH). Human erythrocytes were covalently grafted with LMWH‐SH by the use of a heterobifunctional coupling reagent which reacts with the SH group of LMWH‐SH and surface exposed amino groups of erythrocytes now called ‘heparinocytes’ (HC). HC were morphologically indistinguishable from untreated erythrocytes and displayed identical osmotic resistance. The functionality of HC was analyzed by classical coagulation tests in which they dose dependently inhibited clot formation. HC were also functional in recalcified whole blood inhibiting thrombin formation as assessed by the cleavage of the chromogenic substrate S‐2238. The system appears applicable as a potential autologous, long‐term anticoagulant treatment or prophylaxis.