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ERK activation upon hypoxia: involvement in HIF‐1 activation
Author(s) -
Minet E,
Arnould T,
Michel G,
Roland I,
Mottet D,
Raes M,
Remacle J,
Michiels C
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01181-9
Subject(s) - mapk/erk pathway , hypoxia (environmental) , kinase , transactivation , microbiology and biotechnology , mitogen activated protein kinase 3 , signal transduction , western blot , transcription factor , chemistry , mitogen activated protein kinase , phosphorylation , biology , biochemistry , gene , organic chemistry , oxygen
Hypoxia‐inducible factor‐1 (HIF‐1) is a transcription factor activated by hypoxia. The HIF‐1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF‐1 activation. We demonstrated that in human microvascular endothelial cells‐1 (HMEC‐1), ERK kinases are activated during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed for hypoxia‐induced HIF‐1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF‐1α is phosphorylated in hypoxia by an ERK‐dependent pathway. These results evidence the role of mitogen‐activated protein kinase in the transcriptional response to hypoxia.