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The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non‐homologous end joining
Author(s) -
de la Torre-Ruiz Maria-Angeles,
Lowndes Noel F.
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01180-7
Subject(s) - g2 m dna damage checkpoint , saccharomyces cerevisiae , cell cycle checkpoint , chek1 , non homologous end joining , rad52 , rad51 , dna repair , homologous recombination , microbiology and biotechnology , biology , cell cycle , dna , genetics , gene
In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non‐homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process. Defective NHEJ in rad9 Δ– rad24 Δ, but not yku80 Δ cells, is only partially rescued by imposing G1 or G2/M delays. Thus, checkpoint functions other than transient cell cycle delays may be required for normal levels of NHEJ. Epistasis analysis also indicated that YKU80 and RAD9/RAD24 function in the same pathway for repair of lesions caused by MMS and γ‐irradiation. Unlike NHEJ, the checkpoint pathway is not required for efficient site‐specific integration of plasmid DNA into the yeast genome, which is RAD52 ‐dependent, but RAD51 ‐independent.