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Inhibition of plant telomerase by telomere‐binding proteins from nuclei of telomerase‐negative tissues
Author(s) -
Fulnečková Jana,
Fajkus Jiřı́
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01178-9
Subject(s) - telomerase , telomere , cell division , biology , biochemistry , microbiology and biotechnology , chemistry , cell , dna , gene
The activity of telomerase in plant cells is precisely regulated in response to changes in cell division rate. To explore this regulatory mechanism, the effect on telomerase activity of protein extracts from nuclei of telomerase‐negative tissues was examined. An inhibition of telomerase activity was found which was species‐non‐specific. This inhibition was due to proteins which form salt‐stable, sequence‐specific complexes with the G‐rich telomeric strand and reduce its accessibility, as shown by gel retardation and by terminal transferase (TdT) extension of G‐rich telomeric and non‐telomeric (substrate) primers. A 40 kDa polypeptide was detected by SDS–PAGE after cross‐linking the complex formed by extracts from tobacco leaf nuclei. Such proteins may be involved in regulation of telomerase activity in plants.