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Identification and characterization of functional subunits of Clostridium botulinum type A progenitor toxin involved in binding to intestinal microvilli and erythrocytes
Author(s) -
Fujinaga Yukako,
Inoue Kaoru,
Nomura Takako,
Sasaki Junzo,
Marvaud Jean C.,
Popoff Michel R.,
Kozaki Shunji,
Oguma Keiji
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01147-9
Subject(s) - toxin , clostridium botulinum , hemagglutinin (influenza) , sialic acid , neurotoxin , biochemistry , biology , progenitor , progenitor cell , chemistry , microbiology and biotechnology , stem cell , gene
Clostridium botulinum type A hemagglutinin‐positive progenitor toxin consists of three distinct components: neurotoxin (NTX), hemagglutinin (HA), and non‐toxic non‐HA (NTNH). The HA consists of four subcomponents designated HA1, 2, 3a and 3b. By employing purified toxin and GST‐fusion proteins of each HA subcomponent, we found that the HA‐positive progenitor toxin, GST‐HA1 and GST‐HA3b bind to human erythrocytes and microvilli of guinea pig upper small intestinal sections. The HA‐positive progenitor toxin and GST‐HA1 bind via galactose moieties, GST‐HA3b binds via sialic acid moieties. GST‐2 and GST‐3a showed no detectable binding.