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14‐3‐3τ associates with a translational control factor FKBP12‐rapamycin‐associated protein in T‐cells after stimulation by pervanadate
Author(s) -
Mori Hiroshi,
Inoue Masahiro,
Yano Mihiro,
Wakabayashi Hideki,
Kido Hiroshi
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01126-1
Subject(s) - fkbp , stimulation , chemistry , microbiology and biotechnology , cancer research , medicine , biology , biochemistry
Proteins of the 14‐3‐3 family can associate with and/or modulate the activities of a variety of proteins, such as protooncogene and oncogene products, Cdc25 phosphatases and phosphatidylinositol 3‐kinase, and thus are implicated in regulation of signaling pathways and the cell cycle. We report here that treatment of Jurkat T‐cells with an inhibitor of protein tyrosine phosphatase, pervanadate, induces the association of 14‐3‐3τ with a translational control factor, FKBP12‐rapamycin‐associated protein (FRAP), with significant latter's autophosphorylation. Coimmunoprecipitation of various mutants of FRAP coexpressed with 14‐3‐3τ in COS‐7 cells revealed that 14‐3‐3τ binds to the C‐terminal side of FRAP at unknown site(s) different from the predicted binding motifs to date.