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Myosin light chain phosphorylation‐dependent modulation of volume‐regulated anion channels in macrovascular endothelium
Author(s) -
Nilius Bernd,
Prenen Jean,
Walsh Michael P.,
Carton Iris,
Bollen Mathieu,
Droogmans Guy,
Eggermont Jan
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01097-8
Subject(s) - phosphorylation , myosin light chain kinase , chemistry , immunoglobulin light chain , modulation (music) , myosin , biophysics , biochemistry , biology , physics , antibody , immunology , acoustics
The Rho/Rho‐associated kinase (ROK) pathway has been shown to modulate volume‐regulated anion channels (VRAC) in cultured calf pulmonary artery endothelial (CPAE) cells. Since Rho/ROK can increase myosin light chain phosphorylation, we have now studied the effects of inhibitors of myosin light chain kinase (MLCK) or myosin light chain phosphatase (MLCP) on VRAC in CPAE. Application of ML‐9, an MLCK inhibitor, inhibited VRAC, both when applied extracellularly or when dialyzed into the cell. A similar inhibitory effect was obtained by dialyzing the cells with AV25, a specific MLCK inhibitory peptide. Conversely, NIPP1 191–210 , an MLCP inhibitory peptide, potentiated the activation of VRAC by a 25% hypotonic stimulus. These data indicate that activation of VRAC is modulated by MLC phosphorylation.