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P‐glycoprotein is localized in caveolae in resistant cells and in brain capillaries
Author(s) -
Demeule Michel,
Jodoin Julie,
Gingras Denis,
Béliveau Richard
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01087-5
Subject(s) - caveolae , chinese hamster ovary cell , caveolin 1 , caveolin , glycoprotein , immunoprecipitation , p glycoprotein , microbiology and biotechnology , in vivo , lipid raft , chemistry , caveolin 3 , colchicine , biology , antibody , biochemistry , receptor , signal transduction , immunology , genetics , multiple drug resistance , antibiotics
A significant proportion of P‐glycoprotein (P‐gp) and caveolin was co‐localized in caveolae isolated from resistant (CH R C5) cells overexpressing P‐gp and from drug‐sensitive Chinese hamster ovary cells (AuxB1). The proportion of P‐gp and caveolin associated with caveolar microdomains was higher in CH R C5 cells grown in the presence of P‐gp substrates (cyclosporin A or colchicine) than in untreated CH R C5 cells. Co‐immunoprecipitation of P‐gp and caveolin from CH R C5 lysates suggests that there is a physical interaction between them. Furthermore, co‐localization of P‐gp and caveolin was found in caveolae from brain capillaries, indicating that this association also takes place in vivo.