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Induction of tcI 7 , a gene encoding a β‐subunit of proteasome, in tobacco plants treated with elicitins, salicylic acid or hydrogen peroxide 1
Author(s) -
Etienne Philippe,
Petitot Anne-Sophie,
Houot Valérie,
Blein Jean-Pierre,
Suty Lydie
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01084-x
Subject(s) - salicylic acid , elicitor , systemic acquired resistance , nicotiana tabacum , proteasome , complementary dna , gene , biology , microbiology and biotechnology , protein subunit , biochemistry , chemistry , arabidopsis , mutant
We previously isolated, by differential display and 5′ RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for obacco ryptogein nduced), a gene encoding a β‐subunit of proteasome. Here, we report that tcI 7 was up‐regulated in tobacco plants treated with elicitins (cryptogein and parasiticein) that have been shown to induce a systemic acquired resistance (SAR). Moreover, subsequent inoculation of tobacco with the pathogen Phytophthora parasitica var. nicotianae (Ppn) was shown to induce an additional activation of tcI 7 in tobacco plants pretreated with cryptogein. We also showed an up‐regulation of tcI 7 by salicylic acid (SA). Moreover, accumulation of tcI 7 transcripts after treatment with cryptogein or with SA only occurred in NahG 9 − tobacco plants that do not express the salicylate hydroxylase and thus are able to accumulate SA and develop a SAR. Suppressed accumulation of tcI 7 transcripts in NahG 8 + tobacco plants after cryptogein or SA treatment correlated with the loss of SAR. H 2 O 2 was also shown to up‐regulate tcI 7 in tobacco plants. Using gene walking by PCR we cloned and sequenced the 5′ flanking region of tcI 7 containing hypothetical regulatory sequences, especially myb and NF‐κB boxes, that could be responsible for the regulation of tcI 7 by salicylic acid and H 2 O 2 respectively.