Premium
Gene structure of human cholecystokinin (CCK) type‐A receptor: body fat content is related to CCK type‐A receptor gene promoter polymorphism
Author(s) -
Funakoshi Akihiro,
Miyasaka Kyoko,
Matsumoto Hideo,
Yamamori Shunji,
Takiguchi Sohichi,
Kataoka Kazuhiro,
Takata Yutaka,
Matsusue Kimihiko,
Kono Akira,
Shimokata Hiroshi
Publication year - 2000
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(00)01080-2
Subject(s) - cholecystokinin , cholecystokinin receptor , gene , biology , endocrinology , single nucleotide polymorphism , receptor , medicine , heterozygote advantage , polymorphism (computer science) , microbiology and biotechnology , genetics , genotype
The transcriptional start site of the human cholecystokinin (CCK)‐A receptor gene was determined by the Capsite Hunting method. Two sequence changes were detected, a G to T change in nucleotide −128, and an A to G change in nucleotide −81. The homozygote (T/T, G/G) was detected in 25 of 1296 individuals (1.9%) in the cohort study. This polymorphism showed a significantly higher percent body fat and higher levels of serum insulin and leptin, compared with wild type and heterozygotes. Our study provided the possibility that polymorphism in the promoter region of the CCK‐A receptor gene may be one of genetic factors affecting fat deposition.