A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients | Zendy

Yun Shan Goh | Zendy; Lisa F. P. Ng | Zendy; Laurent Rénia | Zendy

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A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients

Author(s) -

Yun Shan Goh,

Lisa F. P. Ng,

Laurent Rénia

Publication year - 2021

Publication title -

star protocols

Language(s) - English

Resource type - Journals

ISSN - 2666-1667

DOI - 10.1016/j.xpro.2021.100671

Subject(s) - serology , flow cytometry , antibody , covid-19 , virology , spike protein , coronavirus , immunology , biology , medicine , infectious disease (medical specialty) , disease , pathology

Summary One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021) .

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