A qualitative IgG ELISA for detection of SARS-CoV-2-specific antibodies in Syrian hamster serum samples | Zendy

Anita Shete | Zendy; Sreelekshmy Mohandas | Zendy; Rajlaxmi Jain | Zendy; Pragya D. Yadav | Zendy

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A qualitative IgG ELISA for detection of SARS-CoV-2-specific antibodies in Syrian hamster serum samples

Author(s) -

Anita Shete,

Sreelekshmy Mohandas,

Rajlaxmi Jain,

Pragya D. Yadav

Publication year - 2021

Publication title -

star protocols

Language(s) - English

Resource type - Journals

ISSN - 2666-1667

DOI - 10.1016/j.xpro.2021.100573

Subject(s) - hamster , antigen , antibody , virology , biology , covid-19 , microbiology and biotechnology , immunology , medicine , disease , pathology , infectious disease (medical specialty)

This protocol describes an indirect enzyme-linked immunosorbent assay (ELISA) for qualitative detection of IgG antibodies against Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in Syrian hamster serum samples. We describe the preparation of inactivated virus antigen and the negative control antigen, and the use of antigen-coated microtiter plates to detect SARS-CoV-2 specific antibodies from SARS-CoV-2-infected hamsters, including the criteria for differentiating positive vs negative reaction. The limited batch-to-batch variability of this assay has been verified with two batches of independently-prepared antigens.

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