Co-quantification of crAssphage increases confidence in wastewater-based epidemiology for SARS-CoV-2 in low prevalence areas | Zendy

Maxwell L. Wilder | Zendy; Frank A. Middleton | Zendy; David A. Larsen | Zendy; Qian Du | Zendy; Ariana Fenty | Zendy; Teng Zeng | Zendy; Tabassum Z. Insaf | Zendy; Pruthvi Kilaru | Zendy; Mary B. Collins | Zendy; Brittany Kmush | Zendy; Hyatt Green | Zendy

Open Access

Co-quantification of crAssphage increases confidence in wastewater-based epidemiology for SARS-CoV-2 in low prevalence areas

Author(s) -

Maxwell L. Wilder,

Frank A. Middleton,

David A. Larsen,

Qian Du,

Ariana Fenty,

Teng Zeng,

Tabassum Z. Insaf,

Pruthvi Kilaru,

Mary B. Collins,

Brittany Kmush,

Hyatt Green

Publication year - 2021

Publication title -

water research x

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.317

H-Index - 10

ISSN - 2589-9147

DOI - 10.1016/j.wroa.2021.100100

Subject(s) - wastewater , rna , covid-19 , population , biology , virology , environmental science , environmental health , medicine , environmental engineering , genetics , gene , disease , infectious disease (medical specialty)

Highlights• Ultracentrifugation with a sucrose cushion allowed sensitive detection of SARS-CoV-2 wastewater RNA. • Per capita levels of crAssphage were consistent across sites supporting its use as a surrogate for SARS-CoV-2. • Limits of quantification corresponded to daily positive tests as low as 0.37 per 10,000 people, depending on the area. • SARS-CoV-2 RNA signal strength is associated with increased COVID-19 cases in the following 7 days. • SARS-CoV-2:crAssphage ratios were significantly associated with COVID-19 cases.

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