Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro | Zendy

Sontana Siritantikorn | Zendy; Suthatip Jintaworn | Zendy; Sansanee Noisakran | Zendy; Yupin Suputtamongkol | Zendy; Daniel H. Paris | Zendy; Stuart D. Blacksell | Zendy

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Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro

Author(s) -

Sontana Siritantikorn,

Suthatip Jintaworn,

Sansanee Noisakran,

Yupin Suputtamongkol,

Daniel H. Paris,

Stuart D. Blacksell

Publication year - 2012

Publication title -

transactions of the royal society of tropical medicine and hygiene

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.725

H-Index - 105

eISSN - 1878-3503

pISSN - 0035-9203

DOI - 10.1016/j.trstmh.2012.05.004

Subject(s) - orientia tsutsugamushi , enumeration , microbiology and biotechnology , in vitro , biology , virology , scrub typhus , mathematics , genetics , combinatorics

The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.

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