Open AccessApplication of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
Author(s) -
Sontana Siritantikorn,
Suthatip Jintaworn,
Sansanee Noisakran,
Yupin Suputtamongkol,
Daniel Paris,
Stuart D. Blacksell
Publication year - 2012
Publication title -
transactions of the royal society of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.725
H-Index - 105
eISSN - 1878-3503
pISSN - 0035-9203
DOI - 10.1016/j.trstmh.2012.05.004
Subject(s) - orientia tsutsugamushi , enumeration , microbiology and biotechnology , in vitro , biology , virology , scrub typhus , mathematics , genetics , combinatorics
The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.