Open AccessThe influence of GAPT extraction on synapse loss of APPswe/PS1dE9 transgenic mice via adjusting Bcl‐2/Bax balance
Author(s) -
Shi Jing,
Zhang Xuekai,
Ni Jingnian,
Wei Mingqing,
Li Ting,
Zhou Bingling,
Liu Xiawei,
Zhang Liping,
Wang Pengwen,
Tian Jinzhou,
Wang Yongyan
Publication year - 2018
Publication title -
alzheimer's and dementia: translational research and clinical interventions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.49
H-Index - 30
ISSN - 2352-8737
DOI - 10.1016/j.trci.2018.10.008
Subject(s) - synapse , genetically modified mouse , neuron , transgene , apoptosis , morris water navigation task , neuroscience , western blot , donepezil , hippocampus , biology , chemistry , medicine , pathology , dementia , biochemistry , disease , gene
The degeneration of memory‐focused synapses play important roles in Alzheimer's disease (AD) pathogenesis, while it is not well known how β amyloid interferes neuron apoptosis and how a herbal combination GAPT influence synapse loss and neuronal apoptosis pathways of APP/PS1 transgenic mice. Methods Three‐month and six‐month APPswe/PS1dE9 transgenic mice were used. Spatial and memory ability were measured by Morris Water Maze, Neuron and synapse number were assessed by electron microscope; Aβ, Bcl‐2/Bax were determined by immunohistochemistry and western blot. Results APP/PS1 mice not only had increased Aβ accumulation, impaired memory performance, less synapse number, and much more necrosed neurons, but also had significant reduction in the Bcl‐2/Bax ratio. However, GAPT and donepezil showed improved memory performance, less Aβ accumulation, increased neuron and synapse number, as well as restored balance of Bcl‐2/Bax. Discussion GAPT may improve cognitive functions via both reducing Aβ deposition and restoring Bcl‐2/Bax balance of neuron.