Open AccessGeneration of autism spectrum disorder patient-derived iPSC line SDUKIi004-A
Author(s) -
Morad Kamand,
Mirolyuba Ilieva,
Sheena Louise Forsberg,
Mads Thomassen,
Åsa Fex Svenningsen,
Morten Meyer,
Tanja Maria Michel
Publication year - 2020
Publication title -
stem cell research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.654
H-Index - 65
eISSN - 1876-7753
pISSN - 1873-5061
DOI - 10.1016/j.scr.2020.102038
Subject(s) - reprogramming , biology , autism spectrum disorder , induced pluripotent stem cell , germ layer , autism , neurodevelopmental disorder , stem cell , embryonic stem cell , neuroscience , microbiology and biotechnology , bioinformatics , genetics , cell , psychology , psychiatry , gene
Autism is a heterogeneous neurodevelopmental disorder defined by deficits in socialization, communication, and patterns of behavior. Using stem cells to model brain disordersmay yield new understanding about the underlying neuropathological processes and could prove essential for drug development. We present here a newhuman inducedpluripotentstem cell (iPSC) line (SDUKIi004-A) generated from skin fibroblasts derived from a 21-year old male patient diagnosed with Pervasive DevelopmentalDisorder-Not Otherwise Specified (PDD-NOS)("FYNEN-cohort"). Reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.
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