Poly(MAA-PEGMA)-coated superparamagnetic nanoparticles-based magnetic affinity immunoassay for detection of interleukin-6 | Zendy

Xinjun Zhu | Zendy

Open Access

Poly(MAA-PEGMA)-coated superparamagnetic nanoparticles-based magnetic affinity immunoassay for detection of interleukin-6

Author(s) -

Xinjun Zhu

Publication year - 2011

Publication title -

scientia iranica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.299

H-Index - 51

eISSN - 2345-3605

pISSN - 1026-3098

DOI - 10.1016/j.scient.2011.11.034

Subject(s) - immunoassay , horseradish peroxidase , detection limit , monoclonal antibody , chromatography , superparamagnetism , chemistry , linear range , microbiology and biotechnology , antibody , enzyme , biochemistry , medicine , biology , immunology , physics , magnetization , quantum mechanics , magnetic field

Using poly(MAA-PEGMA)-coated superparamagnetic nanoparticles (PMNPs) as carrier, a rapid and sensitive immunoassay for quantitative determination of interleukin-6 (IL-6) has been developed for the first time. This protocol involved a sandwich format in which the IL-6 in samples was first captured by the primary monoclonal antibody (mAb) immobilized on the surface of PMNPs, and then recognized by the second monoclonal antibody labeled with horseradish peroxidase (HRP-mAb). The HRP-mAb dilution and immunoreaction time were examined and optimized. The linear range for IL-6 assay was 3.2–1000 pg mL−1, and the Limit Of Detection (LOD) was 1.0 pg mL−1. The developed immunoassay method showed high precision, high sensitivity, acceptable recovery and relatively good reliability, and could be used to detect serum IL-6 levels with consistent results in comparison with those obtained by the Enzyme-Linked Immunosorbent Assay (ELISA) method

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