Open AccessPoly(MAA-PEGMA)-coated superparamagnetic nanoparticles-based magnetic affinity immunoassay for detection of interleukin-6
Author(s) -
Xinjun Zhu
Publication year - 2011
Publication title -
scientia iranica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.299
H-Index - 51
eISSN - 2345-3605
pISSN - 1026-3098
DOI - 10.1016/j.scient.2011.11.034
Subject(s) - immunoassay , horseradish peroxidase , detection limit , monoclonal antibody , chromatography , superparamagnetism , chemistry , linear range , microbiology and biotechnology , antibody , enzyme , biochemistry , medicine , biology , immunology , physics , magnetization , quantum mechanics , magnetic field
Using poly(MAA-PEGMA)-coated superparamagnetic nanoparticles (PMNPs) as carrier, a rapid and sensitive immunoassay for quantitative determination of interleukin-6 (IL-6) has been developed for the first time. This protocol involved a sandwich format in which the IL-6 in samples was first captured by the primary monoclonal antibody (mAb) immobilized on the surface of PMNPs, and then recognized by the second monoclonal antibody labeled with horseradish peroxidase (HRP-mAb). The HRP-mAb dilution and immunoreaction time were examined and optimized. The linear range for IL-6 assay was 3.2–1000 pg mL−1, and the Limit Of Detection (LOD) was 1.0 pg mL−1. The developed immunoassay method showed high precision, high sensitivity, acceptable recovery and relatively good reliability, and could be used to detect serum IL-6 levels with consistent results in comparison with those obtained by the Enzyme-Linked Immunosorbent Assay (ELISA) method