Open AccessThe role of flavonoid antioxidant, morin in improving procarbazine-induced oxidative stress on testicular function in rat
Author(s) -
Ebenezer Tunde Olayinka,
Ayokanmi Ore,
Oluwatobi Adewumi Adeyemo,
Olaniyi Solomon Ola
Publication year - 2019
Publication title -
porto biomedical journal/porto biomedical journal
Language(s) - English
Resource type - Journals
eISSN - 2444-8672
pISSN - 2444-8664
DOI - 10.1016/j.pbj.0000000000000028
Subject(s) - oxidative stress , glutathione , glutathione peroxidase , antioxidant , sperm , chemistry , superoxide dismutase , lactate dehydrogenase , pharmacology , ascorbic acid , lipid peroxidation , malondialdehyde , sperm motility , biochemistry , medicine , endocrinology , andrology , enzyme , food science
Background: Procarbazine (PCZ) is an effective chemotherapeutic drug used in the treatment of lymphoma; however, oxidative stress–mediated testicular toxicity is a major side effect. Recently, therapeutic intervention using flavonoids against oxidative stress–related pathologies is gaining more attention. Morin (MOR) is a natural flavonoid with proven antioxidant activity. This study was designed therefore to evaluate the potential role of MOR in ameliorating PCZ-induced testicular oxidative stress and altered sperm quality in rat model. Methods: A total of 24 male Wistar rats (170–180 g) were randomly assigned into 4 treatment groups: I, control; II, PCZ (2 mg/kg b.w.); III, PCZ (2 mg/kg b.w.) + MOR (100 mg/kg b.w.) simultaneously administered and IV, MOR (100 mg/kg b.w.), and all treatments lasted 14 days. Results: PCZ treatment displayed significant reduction in sperm number, sperm motility, percentage normal sperm cells, and daily sperm production rate. Meanwhile the activities of testicular enzymes: gamma-glutamyl transferase, acid phosphatase, and lactate dehydrogenase were significantly altered in the PCZ group compared to control. Furthermore, PCZ caused a significant reduction in levels of glutathione and ascorbic acid as well as activities superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase in the testes of PCZ-treated rats. A significant increase in testicular malondialdehyde level was also observed in the PCZ group. MOR treatment, however, significantly restored the altered sperm parameters and biochemical markers in the testis. Conclusions: Our data suggest that MOR administration protected against PCZ-induced testicular and spermatotoxicity in rat, by improving testicular antioxidant system.