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RNAi mediated MMP‐1 silencing inhibits human chondrosarcoma invasion
Author(s) -
Yuan Jun,
Dutton Charyl M.,
Scully Sean P.
Publication year - 2005
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2005.04.004.1100230633
Subject(s) - gene silencing , chondrosarcoma , matrix metalloproteinase , rna interference , metastasis , cancer research , small interfering rna , transfection , osteosarcoma , biology , cell culture , medicine , chemistry , pathology , gene , cancer , rna , genetics
Previous work indicates that matrix metalloproteinase‐1 (MMP‐1) gene expression is a prognostic factor for local recurrence and metastasis in human chondrosarcoma. The current study evaluates the effects of MMP‐1 knock‐down via RNA interference on chondrosarcoma metastasis in vitro. Of nine siRNA oligos tested, one showed a 90% inhibitory effect on MMP‐1 gene expression. Stable attenuation of MMP‐1 in chondrosarcoma cells was achieved by using a plasmid vector‐based siRNA system. Pronounced MMP‐1 activity suppression was accompanied by marked inhibition of tumor cell invasion. Cells in which MMP‐1 has been stably silenced are useful tools to elucidate the mechanism of MMP‐1 mediated tumor metastasis. © 2005 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.