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Gene expression profile of mechanically impacted bovine articular cartilage explants
Author(s) -
Chan P. S.,
Schlueter A. E.,
Coussens P. M.,
Rosa G. J. M.,
Haut R. C.,
Orth M. W.
Publication year - 2005
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2005.01.016
Subject(s) - cartilage , gene expression , microarray , microbiology and biotechnology , microarray analysis techniques , complementary dna , biology , gene , genetics , anatomy
Traumatic injury to a joint can initiate cartilage degradation. Blunt trauma increases matrix damage and decreases proteoglycan synthesis in in vitro models. Few studies have investigated gene expression of articular cartilage (AC) following mechanical loading. Recent advances in microarray technology allow analysis of a number of genes, and may elucidate pathways of AC degradation. In the present study, we used a bovine cDNA microarray to determine how acute trauma of cartilage explants in the absence of underlying bone alters gene expression. Results indicate that at least 19 genes were differentially expressed at 3 h after trauma. Fourteen genes were up‐regulated and five genes were down‐regulated relative to control explants. The up‐regulated genes included cytokine and chemokine receptors, enzymes, and molecules involved in signal transduction. Genes of adhesion molecules and apoptosis were down‐regulated. The results of this study highlight the potential benefits of using a bovine cDNA microarray to study cartilage metabolism. © 2005 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.