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Cytological properties of stromal cells derived from giant cell tumor of bone (GCTSC) which can induce osteoclast formation of human blood monocytes without cell to cell contact
Author(s) -
Nishimura Makoto,
Yuasa Kimitaka,
Mori Kouki,
Miyamoto Noriki,
Ito Morihiro,
Tsurudome Masato,
Nishio Machiko,
Kawano Mitsuo,
Komada Hiroshi,
Uchida Atsumasa,
Ito Yasuhiko
Publication year - 2005
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2005.01.004
Subject(s) - rankl , osteoclast , stromal cell , cell culture , giant cell , monocyte , bone resorption , cytokine , chemistry , tumor necrosis factor alpha , multinucleate , giant cell tumor of bone , macrophage colony stimulating factor , endocrinology , medicine , microbiology and biotechnology , biology , immunology , macrophage , pathology , activator (genetics) , receptor , in vitro , biochemistry , genetics
When human blood monocytes were cocultured with stromal cells derived from human giant cell tumor of bone (GCTSC) and a Millipore filter (0.4 μm) was interposed between monocytes and GCTSC, multinucleated giant cell formation of monocytes was induced. The multinucleated giant cells have characters as osteoclast‐like cells, indicating that a soluble osteoclast‐inducing factor(s) is secreted from GCTSC expressing RANK, RANKL/ODF/OPGL and TACE mRNA. Furthermore, OCIF/OPG inhibited GCTSC‐induced osteoclastogenesis, showing that the RANK–RANKL system is involved in GCTSC‐induced osteoclastogenesis and that soluble form of ODF/RANKL induces osteoclasts from monocytes. GCTSC expressed the cytokine mRNAs such as M‐CSF, GM‐CSF, IL‐3, IL‐4, IL‐6, and IFN‐γ mRNAs. None of IL‐1rα, IL‐1α, IL‐1β, IL‐2, IL‐4, IL‐10, IL‐18, TNF‐α, GCSF and IFN‐γ could be detected in all culture media. A significant amount of IL‐6 could be detected in the culture media of all GCTSC. IL‐8 was found in the culture media of two GCTSC and two osteosarcoma‐derived cells. M‐CSF was detected in all culture media. GCTSC express CaSR, and stimulation of GCTSC with either extracellular Ca 2+ or neomycin, agonist of CaSR, augumented the expression of RANKL. Some lines of GCTSC expressed alkaline phosphatase, osteocalcin and Cbfa1, suggesting that GCTSC are intimately related to osteoblastic lineage. © 2005 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.