Premium
rhBMP‐2, rhVEGF 165 , rhPTN and thrombin‐related peptide, TP508 induce chemotaxis of human osteoblasts and microvascular endothelial cells
Author(s) -
Li Gang,
Cui Yuxin,
McIlmurray Lisa,
Allen William E.,
Wang Hali
Publication year - 2005
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2004.12.005
Subject(s) - angiogenesis , chemotaxis , pleiotrophin , thrombin , microbiology and biotechnology , vascular endothelial growth factor , chemistry , chemotaxis assay , bone remodeling , osteoblast , bone morphogenetic protein 2 , growth factor , biology , immunology , cancer research , endocrinology , platelet , in vitro , biochemistry , receptor , vegf receptors
Osteogenesis and angiogenesis are inter‐linked and tightly regulated processes involved in growth, repair, and bone remodeling. Bone morphogenic protein 2 (BMP‐2), vascular endothelial growth factor (VEGF), pleiotrophin (PTN) and thrombin‐related peptide, TP508 have all been found to have the ability to promote bone fracture healing by enhancing both the osteogenesis and angiogenesis processes. One of the underlying mechanisms proposed is that mediators for osteogenesis may also be involved in mediating angiogenesis and vice versa. The aim of this study was to examine the chemotactic effects of rhBMP‐2, rhVEGF 165 , rhPTN and TP508 on human osteoblasts and endothelial cells. Using a direct‐viewing chemotaxis assay system, we report for the first time, the direct quantitative observation of chemotaxis of both human osteoblastc cells and microvascular endothelial cells towards sources of rhBMP‐2, rhVEGF 165 , rhPTN and TP508. This study confirmed that rhBMP‐2, rhVEGF 165 , rhPTN and TP508 have chemotactic effects on both human osteoblastic and endothelial cells, indicating that these factors are directly involved in promoting angiogenesis and osteogenesis by recruiting osteoblasts and endothelial cells via chemotaxis. © 2005 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.