Premium
Effect of low intensity pulsed ultrasound and BMP‐2 on rat bone marrow stromal cell gene expression
Author(s) -
Sant'Anna Eduardo F.,
Leven Robert M.,
Virdi Amarjit S.,
Sumner D. R.
Publication year - 2005
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2004.09.007
Subject(s) - low intensity pulsed ultrasound , stromal cell , bone marrow , bone morphogenetic protein 2 , cell , gene expression , bone morphogenetic protein 7 , chemistry , microbiology and biotechnology , pathology , gene , ultrasound , bone morphogenetic protein , biology , medicine , therapeutic ultrasound , biochemistry , radiology , in vitro
To determine how low intensity pulsed ultrasound alters gene expression in rat bone marrow stromal cells and to see if combining this stimulation with BMP‐2, cells were pre‐cultured for eight days in the presence of 50μg/ml ascorbic acid and then exposed to either low intensity US or 100ng/ml BMP‐2 or both combined, beginning on the first, third fifth or seventh day of culture so that cells were exposed to the stimuli for one, three, five or seven days. Real time PCR was used to determine the effect of these treatments on gene expression of several genes associated with osteogenesis. The expression of some of the genes (Cbfa‐1/Runx2, IGF‐receptor, Alk‐3, alkaline phosphatase, osteopontin, TGF‐β1, BMP‐7) was increased compared to untreated controls. Combination of US and BMP‐2 treatment did not lead to synergy of the two stimuli. Cbfa‐1 stimulation occurred more quickly with US than with BMP‐2.Increases in gene expression were greatest after 3 days exposure to US, with similar results for BMP‐2 treatment implying that there may be a time dependence for the stimulus of osteogenic gene expression in stromal cells. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.