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Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells
Author(s) -
Chen C. W.,
Tsai Y. H.,
Deng W. P.,
Shih S. N.,
Fang C. L.,
Burch J. G.,
Chen W. H.,
Lai W. F.
Publication year - 2005
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2004.09.002
Subject(s) - aggrecan , chondrogenesis , type ii collagen , extracellular matrix , chemistry , mesenchymal stem cell , progenitor cell , dexamethasone , sox9 , cellular differentiation , microbiology and biotechnology , type i collagen , alkaline phosphatase , transforming growth factor , medicine , glycosaminoglycan , endocrinology , biology , stem cell , gene expression , biochemistry , osteoarthritis , pathology , alternative medicine , enzyme , gene , articular cartilage , arthritis
Chondrogenic differentiation by mesenchymal progenitor cells (MPCs) is associated with cytokines such as transforming growth factor‐beta 1 (TGF‐β1) and dexamethasone. Extracellular matrix (ECM) also regulates the differentiation by MPCs. To define whether ECM plays a functional role in regulation of the chondrogenic differentiation by MPCs, an in vitro model was used. That model exposed to dexamethasone, recombinant human TGF‐β1( rh TGF‐β1) and collagens. The results showed that MPCs incorporated with dexamethasone and rh TGF‐β1 increased proliferation and expression of glycosaminoglycan (GAG) after 14 days. Type II collagen enhanced the GAG synthesis, but did not increase alkaline phosphatase (ALP) activity. When adding dexamethasone and rh TGF‐β1 MPCs increased mRNA expression of Sox9. Incorporation with type II collagen, dexamethasone and rh TGF‐β1, MPCs induced mRNA expression of aggrecan and enhanced levels of type II collagen, and Sox9 mRNA. In contrast, incorporation with type I collagen, dexamethasone and rh TGF‐β1 MPCs reduced levels of aggrecan, and Sox9 mRNA, and showed no type II collagen mRNA. Altogether, these results indicate that type I and II collagen, in addition to the cytokine effect, may play a functional role in regulating of chondrogenic differentiation by MPCs. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.