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Recruitment of osteoclast precursors by stromal cell derived factor‐1 (SDF‐1) in giant cell tumor of bone
Author(s) -
Liao Ted S.,
Yurgelun Matthew B.,
Chang SeongSil,
Zhang HuiZhu,
Murakami Koko,
Blaine Theodore A.,
Parisian May V.,
Kim William,
Winchester Robert J.,
Lee Francis YoungIn
Publication year - 2005
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2004.06.018
Subject(s) - stromal cell , osteoclast , cd34 , giant cell , giant cell tumor of bone , haematopoiesis , bone marrow , cancer research , stem cell , progenitor cell , biology , pathology , chemistry , microbiology and biotechnology , immunology , medicine , receptor
Giant cell tumor (GCT) of bone is a unique bone lesion that is characterized by an excessive number of multinucleated osteoclasts. GCT consists of neoplastic stromal cells, multinucleated osteoclasts and their precursors, thus serving as a naturally occurring human disease model for the study of osteoclastogenesis. It still remains unclear how stromal cells of GCT recruit osteoclast precursors. In the present study, we characterized the cellular components of GCT and confirmed the presence of CD14 + ‐monocytes/CD68 + ‐macrophages and CD34 + ‐hematopoetic stem cells that express CXCR4, a specific receptor for SDF‐1; SDF‐1 gene expression and presence of SDF‐1 protein were confirmed by real time RT‐PCR, in situ hybridization, and immunohistochemistry in the GCT tissue and cultured cells. SDF‐1 was present at 25–50 ng/ml in the conditioned media from the GCT cultures, which is in the range of physiological chemotactic concentration. Migration of osteoclast precursors was 2.5‐fold higher in response to GCT conditioned media compared to the control media; and migration was inhibited by an average of 36% with anti‐SDF‐1 neutralizing antibody or competing recombinant SDF‐1. These results suggest that SDF‐1 is one of the significant chemoattractant factors involved in the recruitment of hematopoietic osteoclast precursor cells during tumor‐induced osteoclastogenesis. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.