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Targeted delivery of progenitor cells for cartilage repair
Author(s) -
Dennis James E.,
Cohen Nir,
Goldberg Victor M.,
Caplan Arnold I.
Publication year - 2004
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2003.12.002
Subject(s) - cartilage , chondrogenesis , progenitor cell , microbiology and biotechnology , matrix (chemical analysis) , chemistry , confocal , chondrocyte , antibody , stem cell , cell , materials science , biology , anatomy , immunology , biochemistry , mathematics , chromatography , geometry
Abstract An approach for promoting the adherence of chondrogenic progenitor cells to specific matrix molecules has been tested in a cartilage defect model. Culture‐expanded pre‐chondrocytes fluorescently labeled with a vital dye were coated by a two‐step method wherein lipidated protein G was first allowed to intercalate into cell membranes, and a second incubation in a solution of antibodies to cartilage matrix antigens allowed the binding of the antibodies to the protein G, on the external surface of the cell. The coating technique (termed „cell painting”) does not effect cell viability or inhibit growth and chondrogenic potential. Painted cells were then added to rabbit cartilage explants that had a partial‐thickness defect, washed, and prepared for histological examination and for confocal microscopy. The histological observations and the confocal observations and fluorescent intensity quantification consistently demonstrated that progenitor cells painted with multiple antibodies were capable of preferential binding to the exposed cartilage matrix within the defect. These results demonstrate that painting cell membranes with antibodies to matrix molecules is an effective method for promoting the adherence of stem or progenitor cells to a cartilage injury site. © 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.