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The use of debrided human articular cartilage for autologous chondrocyte implantation: Maintenance of chondrocyte differentiation and proliferation in type I collagen gels
Author(s) -
Chaipinyo Kanda,
Oakes Barry W.,
Van Damme MariePaule I.
Publication year - 2004
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1016/j.orthres.2003.07.001
Subject(s) - autologous chondrocyte implantation , chondrocyte , cartilage , type ii collagen , articular cartilage repair , sox9 , matrix (chemical analysis) , type i collagen , articular cartilage , proteoglycan , osteoarthritis , chemistry , biomedical engineering , medicine , anatomy , pathology , biochemistry , gene expression , alternative medicine , chromatography , gene
Autologous chondrocyte implantation (ACI) is the most promising surgical treatment for large full thickness knee joint articular cartilage (AC) defects where cells from healthy non‐weight bearing area AC are multiplied in vitro and implanted into such defects. In the routine surgical procedure for symptomatic knee full thickness AC defects, damaged AC surrounding the edge and the base of such defects is usually debrided and discarded. The purpose of this study was to examine if chondrocytes from this ‘debrided’ AC can proliferate, synthesize a cartilage specific matrix and thus can be used for ACI. Methods : Biopsies were retrieved from 12 patients (debrided articular cartilage: DAC, aged 35–61) and from two autopsies (normal articular cartilage: NAC, aged 21 and 25). Chondrocytes were isolated, seeded at low density in type I collagen gels and as monolayer cultures for 4 weeks without passage. Results : After 4 weeks cultures in type I collagen gels, cell proliferation from DAC (18.34±1.95 fold) was similar to cells from NAC (11.24±1.02 fold). Syntheses of proteoglycan and collagen in DAC were also similar to NAC. Newly synthesized matrices in gel cultures consisted predominantly of type II collagen as shown by immuno‐labelling and SDS‐PAGE followed by fluorography. Chondrocytes from ‘debrided human AC’ cultured at low density in type I collagen gels may be used for the ACI procedure as they provide sufficient viable cell numbers for ACI and maintain their chondrocyte phenotype as they synthesize a cartilage‐like matrix. © 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.