Open AccessMTA1 regulates higher‐order chromatin structure and histone H1‐chromatin interaction in‐vivo
Author(s) -
Liu Jian,
Wang Haijuan,
Ma Fei,
Xu Dongkui,
Chang Yanan,
Zhang Jinlong,
Wang Jia,
Zhao Mei,
Lin Chen,
Huang Changzhi,
Qian Haili,
Zhan Qimin
Publication year - 2015
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1016/j.molonc.2014.08.007
Subject(s) - chromatin , scaffold/matrix attachment region , chia pet , interphase , microbiology and biotechnology , biology , bivalent chromatin , histone h1 , chromatin remodeling , nucleosome , histone modifying enzymes , prophase , mitosis , histone , histone code , solenoid , genetics , gene , meiosis , physics , quantum mechanics
In the current study, for the first time, we found that metastasis‐associated gene 1 (MTA1) was a higher‐order chromatin structure organizer that decondenses the interphase chromatin and mitotic chromosomes. MTA1 interacts dynamically with nucleosomes during the cell cycle progression, prominently contributing to the mitotic chromatin/chromosome structure transitions at both prophase and telophase. We showed that the decondensation of interphase chromatin by MTA1 was independent of Mi‐2 chromatin remodeling activity. H1 was reported to stabilize the compact higher‐order chromatin structure through its interaction with DNA. Our data showed that MTA1 caused a reduced H1‐chromatin interaction in‐vivo. Moreover, the dynamic MTA1‐chromatin interaction in the cell cycle contributed to the periodical H1‐chromatin interaction, which in turn modulated chromatin/chromosome transitions. Although MTA1 drove a global decondensation of chromatin structure, it changed the expression of only a small proportion of genes. After MTA1 overexpression, the up‐regulated genes were distributed in clusters along with down‐regulated genes on chromosomes at parallel frequencies.