Open AccessCharacterizing DNA methylation patterns in pancreatic cancer genome
Author(s) -
Tan Aik Choon,
Jimeno Antonio,
Lin Steven H.,
Wheelhouse Jenna,
Chan Fonda,
Solomon Anna,
Rajeshkumar N.V.,
Rubio-Viqueira Belen,
Hidalgo Manuel
Publication year - 2009
Publication title -
molecular oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.332
H-Index - 88
eISSN - 1878-0261
pISSN - 1574-7891
DOI - 10.1016/j.molonc.2009.03.004
Subject(s) - dna methylation , pancreatic cancer , methylation , biology , epigenetics , cpg site , cancer research , illumina methylation assay , gene , cancer , microbiology and biotechnology , genetics , gene expression
We performed a global methylation profiling assay on 1505 CpG sites across 807 genes to characterize DNA methylation patterns in pancreatic cancer genome. We found 289 CpG sites that were differentially methylated in normal pancreas, pancreatic tumors and cancer cell lines. We identified 23 and 35 candidate genes that are regulated by hypermethylation and hypomethylation in pancreatic cancer, respectively. We also identified candidate methylation markers that alter the expression of genes critical to gemcitabine susceptibility in pancreatic cancer. These results indicate that aberrant DNA methylation is a frequent epigenetic event in pancreatic cancer; and by using global methylation profiling assay, it is possible to identify these markers for diagnostic and therapeutic purposes in this disease.